Rapid detection ofbordetella pertussis by real-time PCR using SYBR green I and a LightCycler instrument
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چکیده
منابع مشابه
A real-time PCR assay for the detection and quantitation of Campylobacter jejuni using SYBR Green I and the LightCycler.
Campylobacter jejuni is recognized as a leading human food-borne pathogen. Traditional biochemical identification for C. jejuni is not reliable due to special growth requirements and the possibility that this bacterium can enter a viable but nonculturable (VNC) state. Nucleic acid-based tests have emerged as a useful alternative to traditional testing. In this article, we present fluorescent qu...
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The present study was envisaged to compare the sensitivity of SYBR Green real time PCR with immunofluorescence PCR for diagnosis of rabies. SYBR Green real time PCR technique was applied on brain samples collected from 39 rabies suspected animals. Sensitivity of SYBR Green technique was compared in accordance with WHO recommended gold standard test viz. Fluorescent Antibody Technique (FAT) appl...
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Background and purpose: There are various methods for molecular detection of SARS-CoV2 genome among which, PCR-based methods are the most reliable for making diagnosis. The majority of approved PCR kits for detection of Coronavirus are based on TaqMan real-time PCR which is expensive due to incorporating fluorescent and quencher-harboring probe. The aim of this study was to design a simple and ...
متن کاملQuantitative detection of chicken meat routine mislabeling in emulsion type sausages and burgers by SYBR green real time PCR assay
ABSTRACT- Today, the authenticity of meat products with less costly and desirable species has increased. Therefore and considering religious, economicalor public health concerns, proper actions should be taken to prevent such frauds. In this study, real time PCR assay was applied for rapid, sensitive and specific identification and quantification of chicken tissue in meat products. Specific pri...
متن کاملDevelopment of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis
Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tube...
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ژورنال
عنوان ژورنال: Journal of Clinical Laboratory Analysis
سال: 2004
ISSN: 0887-8013,1098-2825
DOI: 10.1002/jcla.20035